Clinical Microbiology Newsletter
Volume 30, Issue 6 , Pages 39-45 , 15 March 2008

Development and Utilization of West Nile Virus Antibody Assays in a Reference Laboratory Setting

  • Harry E. Prince, Ph.D., D(ABMLI)

      Affiliations

    • Corresponding Author InformationMailing Address: Harry E. Prince, Ph.D, D(ABMLI), Focus Diagnostics, Cypress, CA 90630. Tel.: 714-503-2047
    • ,
  • Mary Lapé-Nixon, M.S.

References 

  1. Petersen LR , Marfin AA . West Nile virus: a primer for the clinician . Ann. Intern. Med. . 2002;137:173–179
  2. Smithburn KC , et al.   Neurotropic virus isolated from blood of a native of Uganda . Am. J. Trop. Med. . 1940;20:471–492
  3. Sampathkumar P . West Nile virus: epidemiology, clinical presentation, diagnosis, and prevention . Mayo Clinic Proc. . 2003;78:1137–1144
  4. Petersen LR , Roehrig JT , Hughes JM . West Nile virus encephalitis . N. Engl. J. Med. . 2002;347:1225–1226
  5. Sevjar JJ , et al.   Acute flaccid paralysis and West Nile virus infection . Emerg. Infect. Dis. . 2003;9:788–793
  6. Centers for Disease Control and Prevention West Nile virus website . http://www.cdc.gov/ncidod/dvbid/westnile/surv&control.htm Accessed 11 October 2007.
  7. Tardei G , et al.   Evaluation of Immunoglobulin M (IgM) and IgG enzyme immunoassays in serologic diagnosis of West Nile virus infection . J. Clin. Microbiol. . 2000;38:2232–2239
  8. Johnson AJ , et al.   Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay . J. Clin. Microbiol. . 2000;38:1827–1831
  9. Prince HE , et al.   Development and persistence of West Nile virus-specific immunoglobulin M (IgM), IgA, and IgG in viremic blood donors . J. Clin. Microbiol. . 2005;43:4316–4320
  10. Prince HE , Hogrefe WR . Performance characteristics of an in-house assay system used to detect West Nile virus (WNV)-specific immunoglobulin M during the 2001 WNV season in the United States . Clin. Diagn. Lab. Immunol. . 2003;10:177–179
  11. Prince HE , Hogrefe WR . Detection of West Nile virus (WNV)-specific immunoglobulin M in a reference laboratory setting during the 2002 WNV season in the United States . Clin. Diagn. Lab. Immunol. . 2003;10:764–768
  12. Hogrefe WR , et al.   Performance of immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays using a West Nile virus recombinant antigen (preM/E) for detection of West Nile virus- and other flavivirus-specific antibodies . J. Clin. Microbiol. . 2004;42:4641–4648
  13. Prince HE , et al.   Utility of the Focus Technologies West Nile virus Immunoglobulin M capture enzyme-linked immunosorbent assay for testing cerebrospinal fluid . J. Clin. Microbiol. . 2004;42:12–15
  14. Roehrig JT , et al.   Persistence of virus-reactive serum immunoglobulin M antibody in confirmed West Nile virus encephalitis cases . Emerg. Infect. Dis. . 2003;9:376–379
  15. Lanciotti RS . 2004. Current laboratory protocols for West Nile virus. Centers for Disease Control and Prevention West Nile Virus website . http://www.cdc.gov/ncidod/dvbid/westnile/conf/February_2004.htm Accessed 11 May 2004.
  16. Prince HE , Lapé-Nixon M . Evaluation of a West Nile virus immunoglobulin A capture enzyme-linked immunosorbent assay . Clin. Diagn. Lab. Immunol. . 2005;12:231–233
  17. Lapé-Nixon M , Prince HE . West Nile virus immunoglobulin A (IgA) detection in cerebrospinal fluid in relation to WNV IgG and IgM reactivity . J. Clin. Virol. . 2006;37:174–178
  18. Busch MP , et al.   West Nile virus infections projected from blood donor screening data, United States, 2003 . Emerg. Infect. Dis. . 2006;12:395–402
  19. Prince, H.E. et al. Persistence of West Nile virus-specific antibodies in viremic blood donors. Clin. Vaccine Immunol. 14: 1228 1230.
  20. Bodeus M , Feyder S , Goubau P . Avidity of IgG antibodies distinguishes primary from nonprimary cytomegalovirus infection in pregnant women . Clin. Diagn. Lab. Immunol. . 1998;9:9–16
  21. Bottinger B , Jensen IP . Maturation of rubella IgG avidity over time after acute rubella infection . Clin. Diagn. Virol. . 1997;8:105–111
  22. Prince HE , Leber AL . Validation of an in-house assay for cytomegalovirus immunoglobulin G (CMV IgG) avidity and relationship of avidity to CMV IgM levels . Clin. Diagn. Lab. Immunol. . 2002;9:824–827
  23. Prince HE , et al.   Utilization of follow-up specimens from viremic blood donors to assess the value of West Nile virus immunoglobulin G avidity as an indicator of recent infection . Clin. Diagn. Lab. Immunol. . 2005;12:1123–1126
  24. Fox JL , et al.   Immunoglobulin G avidity in differentiation between early and late antibody responses to West Nile virus . Clin. Vaccine Immunol. . 2006;13:33–36
  25. Wong SJ , et al.   Immunoassay targeting nonstructural protein 5 to differentiate West Nile virus infection from dengue and St. Louis encephalitis virus infections and from flavivirus vaccination . J. Clin. Microbiol. . 2003;41:4217–4223

PII: S0196-4399(08)00013-5

doi: 10.1016/j.clinmicnews.2008.02.004

Clinical Microbiology Newsletter
Volume 30, Issue 6 , Pages 39-45 , 15 March 2008

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