Clinical Microbiology Newsletter
Volume 30, Issue 23 , Pages 175-179 , 1 December 2008

GeneXpert Testing: Applications for Clinical Microbiology, Part I*

  • Elizabeth M. Marlowe, Ph.D., D(ABMM)

      Affiliations

    • Southern California Permanente Medical Group, Regional Reference Laboratories, North Hollywood, California
    • Corresponding Author InformationMailing Address: Elizabeth M. Marlowe, Ph.D., D(ABMM), Southern California Permanente Medical Group, Regional Reference Laboratories, North Hollywood, CA 91605. Tel.: 818-503-7067. Fax: 818-503-6713
  • ,
  • Donna M. Wolk, Ph.D., D(ABMM)

      Affiliations

    • Department of Pathology, University of Arizona, College of Medicine, BIO5 Institute, Tucson, Arizona

References 

  1. Edwards RK , et al.   Rapid group B streptococci screening using a real-time polymerase chain reaction assay . Obstet. Gynecol. . 2008;111:1335–1341
  2. Gavino M , Wang E . A comparison of a new rapid real-time polymerase chain reaction system to traditional culture in determining group B streptococcus colonization . Am. J. Obstet. Gynecol. . 2007;197:384–388
  3. Kost CB , et al.   Multicenter beta trial of the GeneXpert enterovirus assay . J. Clin. Microbiol. . 2007;45:1081–1086
  4. Marlowe EM , et al.   Performance of the GeneXpert enterovirus assay for detection of enteroviral RNA in cerebrospinal fluid . J. Clin. Virol. . 2008;43:110–113
  5. Cepheid  . Xpert MRSA package insert . Sunnyvale, CA: Cepheid; 2007;
  6. Bergeron MG , et al.   Rapid detection of group B streptococci in pregnant women at delivery . N. Engl. J. Med. . 2000;343:175–179
  7. Schrag SJ , et al.   A population-based comparison of strategies to prevent early-onset group B streptococcal disease in neonates . N. Engl. J. Med. . 2002;347:233–239
  8. Schrag S , et al.   Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC . Morb. Mortal. Wkly. Rep. Recomm. Rep. . 2002;51:1–22
  9. Schrag SJ , et al.   Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis . N. Engl. J. Med. . 2000;342:15–20
  10. Stapleton RD , et al.   Risk factors for group B streptococcal genitourinary tract colonization in pregnant women . Obstet. Gynecol. . 2005;106:1246–1252
  11. Annonymous  . Disparities in universal prenatal screening for group B streptococcus –North Carolina, 2002-2003 . MMWR Morb. Mortal. Wkly. Rep. . 2005;54:700–703
  12. Anonymous  . Perinatal group B streptococcal disease after universal screening recomm endations – United States, 2003-2005 . MMWR Morb. Mortal. Wkly. Rep. . 2007;56:701–705
  13. Honest H , Sharma S , Khan KS . Rapid tests for group B Streptococcus colonization in laboring women: a systematic review . Pediatrics . 2006;117:1055–1066
  14. Yancey MK , et al.   The accuracy of late antenatal screening cultures in predicting genital group B streptococcal colonization at delivery . Obstet. Gynecol. . 1996;88:811–815
  15. De Cueto M , et al.   Timing of intrapartum ampicillin and prevention of vertical transmission of group B streptococcus . Obstet. Gynecol. . 1998;91:97–102
  16. Davies HD , et al.   Multicenter study of a rapid molecular-based assay for the diagnosis of group B streptococcus colonization in pregnant women . Clin. Infect. Dis. . 2004;39:1129–1135
  17. Cepheid  . Smart GBS assay package insert . Sunnyvale, CA: Cepheid; 2007;
  18. Stanway GF , et al.   Picornaviridae . In:  Fauquet CM , et al. editor. Virus taxonomy. 8th report of the International Committee on the Taxonomy of Viruses . London, United Kingdom: Elsevier Academic Press; 2005;p. 757–778
  19. Rotbart HA . Enteroviral infections of the central nervous system . Clin. Infect. Dis. . 1995;20:971–981
  20. Rotbart HA , et al.   Clinical significance of enteroviruses in serious summer febrile illnesses of children . Pediatr. Infect. Dis. J. . 1999;18:869–874
  21. Strikas RA , Anderson LJ , Parker RA . Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983 . J. Infect. Dis. . 1986;153:346–351
  22. Ginocchio CC , et al.   Development, technical performance, and clinical evaluation of a NucliSens basic kit application for detection of enterovirus RNA in cerebrospinal fluid . J. Clin. Microbiol. . 2005;43:2616–2623
  23. Storch GA . Central nervous System Infections . In:  Storch GA editors. Essentials of diagnostic virology . New York: Churchhill Livingstone; 2000;p. 37–58
  24. Capaul SE , Gorgievski-Hrisoho M . Detection of enterovirus RNA in cerebrospinal fluid (CSF) using NucliSens EasyQ Enterovirus assay . J. Clin. Virol. . 2005;32:236–240
  25. Lai KK , et al.   Evaluation of real-time PCR versus PCR with liquid-phase hybridization for detection of enterovirus RNA in cerebrospinal fluid . J. Clin. Microbiol. . 2003;41:3133–3141
  26. Landry ML , Garner R , Ferguson D . Real-time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens . J. Clin. Microbiol. . 2005;43:3136–3139
  27. Monpoeho S , et al.   Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid . Eur. J. Clin. Microbiol. Infect. Dis. . 2002;21:532–536
  28. Petitjean J , et al.   Development and evaluation of a real-time RT-PCR assay on the LightCycler for the rapid detection of enterovirus in cerebrospinal fluid specimens . J. Clin. Virol. . 2006;35:278–284
  29. Silekens, P. et al. Real time NASBA for the detection of enterovirus RNA in cerebrospinal fluid, abstr. S14. Pan American Society for Clinical Virology Meeting, Clearwater, FL.
  30. Verstrepen WA , et al.   Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay . J. Clin. Microbiol. . 2001;39:4093–4096
  31. Nigrovic LE , Chiang VW . Cost analysis of enteroviral polymerase chain reaction in infants with fever and cerebrospinal fluid pleocytosis . Arch. Pediatr. Adolesc. Med. . 2000;154:817–821
  32. Polage CR , Petti CA . Assessment of the utility of viral culture of cerebrospinal fluid . Clin. Infect. Dis. . 2006;43:1578–1579
  33. Ramers C , et al.   Impact of a diagnostic cerebrospinal fluid enterovirus polymerase chain reaction test on patient management . JAMA . 2000;283:2680–2685
  34. Robinson CC , et al.   Impact of rapid polymerase chain reaction results on management of pediatric patients with enteroviral meningitis . Pediatr. Infect. Dis. J. . 2002;21:283–286
  35. Stellrecht KA , et al.   The impact of an enteroviral RT-PCR assay on the diagnosis of aseptic meningitis and patient management . J. Clin. Virol. . 2002;25(Suppl.1):S19–S26
  36. Hamilton MS , et al.   Clinical utility of polymerase chain reaction testing for enteroviral meningitis . Pediatr. Infect. Dis. J. . 1999;18:533–537
  37. van Vliet KE , et al.   Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses . J. Clin. Microbiol. . 2001;39:3390–3392
  38. Cepheid  . Xpert EV package insert . Sunnyvale, CA: Cepheid; 2006;
  39. Seme K , et al.   GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels . J. Clin. Microbiol. . 2008;46:1510–1513
  40. Bernard H.C., B. Starkley, and B. Yen-Lieberman. 2007. Evaluation of enterovirus detection using the GeneXpert Dx system, abstr. C-085. Abstr. 107th Gen. Meet. Am. Soc. Microbiol., American Society for Microbiology, Washington, DC.
  41. Klevens RM , et al.   Invasive methicillin-resistant Staphylococcus aureus infections in the United States . JAMA . 2007;298:1763–1771
  42. Anonymous  . National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2003, issued August 2003 . Am. J. Infect. Control . 2003;31:481–498
  43. Edwards JR , et al.   National Healthcare Safety Network (NHSN) Report, data summary for 2006, issued June 2007 . Am. J. Infect. Control . 2007;35:290–301
  44. Muto CA , et al.   SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylo coccus aureus and enterococcus . Infect. Contr. Hosp. Epidemiol. . 2003;24:362–386
  45. McKibben L , et al.   Ensuring rational public reporting systems for health care-associated infections: systematic literature review and evaluation recommendations . Am. J. Infect. Control . 2006;34:142–149
  46. McKibben L , et al.   Guidance on public reporting of healthcare-associated infections: recommendations of the Healthcare Infection Control Practices Advisory Committee . Am. J. Infect. Control . 2005;33:217–226
  47. Siegel JD , et al.   Management of multidrug-resistant organisms in health care settings, 2006 . Am. J. Infect. Control . 2007;35:S165–S193

 *Editor's Note: Part II of this article will be published in the December 15, 2008 issue of CMN (Vol. 30, No. 24).NOTE: No responsibility is assumed by the Publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. No suggested test or procedure should be carried out unless, in the reader's judgment, its risk is justified. Because of rapid advances in the medical sciences, we recommend that the independent verification of diagnoses and drug doses should be made. Discussions, views and recommendations as to medical procedures, choice of drugs and drug dosages are the responsibility of the authors.SUBSCRIPTION INFORMATION: Access to full text online is included in the price of individual print subscriptions. Please visit www.cmnewsletter.com. Clinical Microbiology Newsletter (ISSN 0196-4399) is issued twice monthly in one indexed volume per year by Elsevier, 360 Park Avenue South, New York, NY 10010. Subscription price per year: Personal: U.S., $95; International, $101. Institutional: U.S., $559; Interational, $558. Periodical postage paid at New York, NY and at additional mailing offices. Postmaster: Send address changes to Clinical Microbiology Newsletter, Elsevier Science Inc., 360 Park Avenue South, New York, NY 10010. For customer service, phone (212) 633-3950; TOLL-FREE for customers in the United States and Canada: 1-888-4ES-INFO (1888-437-4636) or fax: (212) 633-3860.Reprints: For copies of 100 more of articles in this publication, please contact the Commercial Reprints Department, Elsevier Science, Inc., 360 Park Avenue South, New York, NY 10010-1710. Tel. (212) 633-3813, Fax: (212) 633-3820, e-mail: reprints@elsevier.com

PII: S0196-4399(08)00056-1

doi: 10.1016/j.clinmicnews.2008.11.001

Clinical Microbiology Newsletter
Volume 30, Issue 23 , Pages 175-179 , 1 December 2008