Clinical Microbiology Newsletter
Volume 30, Issue 24 , Pages 183-188 , 15 December 2008

GeneXpert Testing: Applications for Clinical Microbiology, Part II

  • Elizabeth M. Marlowe, Ph.D., D(ABMM)

      Affiliations

    • Southern California Permanente Medical Group, Regional Reference Laboratories, North Hollywood, California
    • Corresponding Author InformationMailing Address: Elizabeth M. Marlowe, Ph.D., D(ABMM), Southern California Permanente Medical Group, Regional Reference Laboratories, North Hollywood, CA 91605. Tel.: 818-503-7067. Fax: 818-503-6713
    • ,
  • Donna M. Wolk, Ph.D., D(ABMM)

      Affiliations

    • Department of Pathology, University of Arizona, College of Medicine, BIO5 Institute, Tucson, Arizona

References 

  1. Conterno LO , et al.   Real-time polymerase chain reaction detection of methicillin-resistant Staphylococcus aureus: impact on nosocomial transmission and costs . Infect. Contr. Hosp. Epidemiol. . 2007;28:1134–1141
  2. Lee S , et al.   Comparison of protocols for surveillance of methicillin-resistant Staphylococcus aureus (MRSA): medical staff vs. ICU patients . Ann. Clin. Lab. Sci. . 2007;37:248–250
  3. Peterson LR , Hacek DM , Robicsek A . Case study: an MRSA intervention at Evanston Northwestern Healthcare . Jt. Comm. J. Qual. Patient Safety . 2007;33:732–738
  4. Regan JA , et al.   Colonization with group B streptococci in pregnancy and adverse outcome. VIP Study Group . Am. J. Obstet. Gynecol. . 1996;174:1354–1360
  5. Richmond I , et al.   Best-practice protocols: reducing harm from MRSA . Nurs. Manage. . 2007;38:22–27
  6. van Trijp MJ , et al.   Successful control of widespread methicillin-resistant Staphylococcus aureus colonization and infection in a large teaching hospital in the Netherlands . Infect. Contr. Hosp. Epidemiol. . 2007;28:970–975
  7. Diekema DJ , Edmond MB . Look before you leap: active surveillance for multidrug-resistant organisms . Clin. Infect. Dis. . 2007;44:1101–1107
  8. Walsh TJ , et al.   Prospective microbiologic surveillance in control of nosocomial methicillin-resistant Staphylococcus aureus . Infect. Contr. . 1987;8:7–14
  9. Blanc DS , et al.   Molecular epidemiology of predominant clones and sporadic strains of methicillin resistant Staphylococcus aureus in Switzerland and comparison with European epidemic clones . Clin. Microbiol. Infect. . 2002;8:419–426
  10. Dias CG , et al.   Use of a novel selective medium to detect methicillin-resistant Staphylococcus aureus in colonized patients of an intensive care unit . Infect. Contr. Hosp. Epidemiol. . 2004;25:130–132
  11. Khoury J , et al.   Eradication of methicillin-resistant Staphylococcus aureus from a neonatal intensive care unit by active surveillance and aggressive infection control measures . Infect. Contr. Hosp. Epidemiol. . 2005;26:616–621
  12. Smyth RW , Kahlmeter G . Mannitol salt agar-cefoxitin combination as a screening medium for methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2005;43:3797–3799
  13. Cherkaoui A , et al.   Comparison of four chromogenic media for culture-based screening of methicillin-resistant Staphylococcus aureus . J. Med. Microbiol. . 2007;56:500–503
  14. Compernolle V , Verschraegen G , Claeys G . Combined use of Pastorex Staph-Plus and either of two new chromogenic agars, MRSA ID and CHROMagar MRSA, for detection of methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2007;45:154–158
  15. Diederen B , et al.   Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2005;43:1925–1927
  16. Flayhart D , et al.   Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares . J. Clin. Microbiol. . 2005;43:5536–5540
  17. Han Z , et al.   Evaluation of mannitol salt agar, CHROMagar Staphylococcus aureus and CHROMagar MRSA for detection of methicillin-resistant Staphylococcus aureus from nasal swab specimens . J. Med. Microbiol. . 2007;56:43–46
  18. Hedin G , Fang H . Evaluation of two new chromogenic media, CHROMagar MRSA and S. aureus ID, for identifying Staphylococcus aureus and screening methicillin-resistant S. aureus . J. Clin. Microbiol. . 2005;43:4242–4244
  19. Lagace-Wiens PR , et al.   Reduction in workload and reporting time of MRSA screening with MRSASelect medium versus mannitol-salt medium supplemented with oxacillin . J. Clin. Microbiol. . 2008;46:1174–1177
  20. Nahimana I , Francioli P , Blanc DS . Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus . Clin. Microbiol. Infect. . 2006;12:1168–1174
  21. Perry JD , et al.   Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2004;42:4519–4523
  22. Stoakes L , et al.   Prospective comparison of a new chromogenic medium, MRSASelect, to CHROMagar MRSA and mannitol-salt medium supplemented with oxacillin or cefoxitin for detection of methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2006;44:637–639
  23. Taguchi H , et al.   Evaluation of a new chromogenic medium for isolation of MRSA . Kansenshogaku Zasshi . 2004;78:54–58
  24. van Loo IHM , et al.   Evaluation of a chromogenic agar (MRSASelect) for the detection of methicillin-resistant Staphylococcus aureus with clinical samples in The Netherlands . J. Med. Microbiol. . 2007;56:491–494
  25. Marx J.L., et al. Comparison of BioRad MRSASelect Agar and BD CHROMagar MRSA for recovery of MRSA from clinical specimens, abstr. C-127. Abstr. 108th Gen. Meet., Am. Soc. Microbiol. American Society for Microbiology, Washington, DC.
  26. Remel, Inc.  . Spectra MRSA Package Insert . Lenexa, KS: Remel; 2007;
  27. Araj GF , et al.   Discrepancies between mecA PCR and conventional tests used for detection of methicillin resistant Staphylococcus aureus . Int. J. Antimicrob. Agents . 1999;11:47–52
  28. Francois P , et al.   Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay . J. Clin. Microbiol. . 2003;41:254–260
  29. Donnio PY , et al.   Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals . Antimicrob. Agents Chemother. . 2007;51:4342–4350
  30. Francois P , et al.   Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2007;45:2011–2013
  31. Francois P , et al.   Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993–2005 . Emerg. Infect. Dis. . 2008;14:304–307
  32. Harbarth S , et al.   Community-associated methicillin-resistant Staphylococcus aureus, Switzerland . Emerg. Infect. Dis. . 2005;11:962–965
  33. Moran GJ , et al.   Methicillin-resistant S. aureus infections among patients in the emergency department . N. Engl. J. Med. . 2006;355:666–674
  34. Ito T , Kuwahara K , Hiramatsu K . Staphylococcal cassette chromosome mec(SCC mec) analysis of MRSA . Methods Mol. Biol. . 2007;391:87–102
  35. Ito T , et al.   Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC . Drug Resist. Updat. . 2003;6:41–52
  36. Kondo Y , et al.   Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions . Antimicrob. Agents Chemother. . 2007;51:264–274
  37. Huletsky A , et al.   New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci . J. Clin. Microbiol. . 2004;42:1875–1884
  38. Bishop EJ , et al.   Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus . J. Clin. Microbiol. . 2006;44:2904–2908
  39. Boyce JM , Havill NL . Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains . J. Clin. Microbiol. . 2008;46:350–351
  40. Cunningham R , et al.   Effect on MRSA transmission of rapid PCR testing of patients admitted to critical care . J. Hosp. Infect. . 2007;65:24–28
  41. de Souza Luna LK , et al.   Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens . J. Clin. Microbiol. . 2007;45:1098–1101
  42. Drews SJ , et al.   Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting . J. Clin. Microbiol. . 2006;44:3794–3796
  43. Farley JE , et al.   Comparison of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR Assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population . J. Clin. Microbiol. . 2008;46:743–746
  44. Francois P , et al.   A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements . J. Clin. Microbiol. . 2004;42:3309–3312
  45. Huletsky A , et al.   Identification of methicillin-resistant Staphylococcus aureus carriage in less than 1 hour during a hospital surveillance program . Clin. Infect. Dis. . 2005;40:976–981
  46. Jeyaratnam D , et al.   Validation of the IDI-MRSA system for use on pooled nose, axilla, and groin swabs and single swabs from other screening sites . Diagn. Microbiol. Infect. Dis. . 2008;61:1–5
  47. Liassine N , Decosterd F , Etienne J . Evaluation of IDI-MRSA assay on a collection of community-acquired methicillin-resistant Staphylococcus aureus isolates and on carriage specimens . Pathol. Biol. . 2007;55:378–381
  48. Rossney AS , et al.   Evaluation of the IDI-MRSA assay on the SmartCycler real-time PCR platform for rapid detection of MRSA from screening specimens . Eur. J. Clin. Microbiol. Infect. Dis. . 2007;26:459–466
  49. van Hal SJ , et al.   Methicillin-resistant Staphylococcus aureus (MRSA) detection: comparison of two molecular methods (IDI-MRSA PCR assay and GenoType MRSA Direct PCR assay) with three selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for use with infection-control swabs . J. Clin. Microbiol. . 2007;45:2486–2490
  50. Zhang SX , et al.   Comparison of two versions of the IDI-MRSA assay using charcoal swabs for prospective nasal and nonnasal surveillance samples . J. Clin. Microbiol. . 2007;45:2278–2280
  51. Warren DK , et al.   Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay . J. Clin. Microbiol. . 2004;42:5578–5581
  52. Rossney AS , et al.   Evaluation of the Xpert MRSA assay on the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens . J. Clin. Microbiol. . 2008;46:3285–3290
  53. Katayama Y , et al.   Genetic background affects stability of mecA in Staphylococcus aureus . J. Clin. Microbiol. . 2005;43:2380–2383
  54. Picton, E. et al. 2008. Rapid detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from wound specimens and blood cultures, abstr. C-083. Abstr. 108th Gen. Meet., Am. Soc. Microbiol. American Society for Microbiology, Washington, DC.

 Editor's Note: Part I of this article was published in the December 1, 2008 issue of CMN (Vol. 30, No. 23).

PII: S0196-4399(08)00059-7

doi: 10.1016/j.clinmicnews.2008.11.004

Clinical Microbiology Newsletter
Volume 30, Issue 24 , Pages 183-188 , 15 December 2008

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